Coding

Part:BBa_M45424:Design

Designed by: Richard Klein   Group: UtahState_BE5930_-_S14   (2018-04-25)


Alkane hydroxylase (Pseudomonas oleovorans)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1068
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097


Design Notes

Two nucleotides needed to be altered to eliminate EcoRI and SpeI restriction endonuclease sites from the IDT optimized protein coding sequence.


Source

The amino acid sequence for this alkane hydroxylase came from Pseudomonas oleovorans and was optimized for expression in E. coli using IDT codon optimization.

References